Mapping small molecule binding sites with phage display technology [electronic resource] / Lee Makowski.
Material type:
FilmSeries: Henry Stewart talksBiomedical & life sciences collection. Chemical biology : the role of chemistry in our fundamental understanding of biology: Publisher: London : Henry Stewart Talks, 2008Description: 1 online resource (1 streaming video file (39 min.) : color, sound)Subject(s): Biology | ChemistryOnline resources: Click here to access online | Series Animated audio-visual presentation with synchronized narration.
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Contents: How do you identify the molecular target of a small molecule drug? -- Flexible regions are key players -- P-loop of ATP-binding proteins is an important example -- Can display libraries of flexible peptides on proteins select those that bind to the ligand -- Selection is through an affinity process called biopanning -- Success depends on the diversity of the library -- Diversity depends on how many different peptides are in the library and their relative abundance -- RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides -- Identification of motifs from a population is easy if the motif is very strong -- Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein -- Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol.
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Mode of access: World Wide Web.