Makowski, Lee.
Mapping small molecule binding sites with phage display technology [electronic resource] / Lee Makowski. - London : Henry Stewart Talks, 2008. - 1 online resource (1 streaming video file (39 min.) : color, sound). - Chemical biology : the role of chemistry in our fundamental understanding of biology, 2056-452X . - Henry Stewart talks. Biomedical & life sciences collection. Chemical biology : the role of chemistry in our fundamental understanding of biology. .
Animated audio-visual presentation with synchronized narration. Title from title frames.
Contents: How do you identify the molecular target of a small molecule drug? -- Flexible regions are key players -- P-loop of ATP-binding proteins is an important example -- Can display libraries of flexible peptides on proteins select those that bind to the ligand -- Selection is through an affinity process called biopanning -- Success depends on the diversity of the library -- Diversity depends on how many different peptides are in the library and their relative abundance -- RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides -- Identification of motifs from a population is easy if the motif is very strong -- Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein -- Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol.
Access restricted to subscribers.
Mode of access: World Wide Web.
1717 Henry Stewart Talks
Biology.
Chemistry.
Mapping small molecule binding sites with phage display technology [electronic resource] / Lee Makowski. - London : Henry Stewart Talks, 2008. - 1 online resource (1 streaming video file (39 min.) : color, sound). - Chemical biology : the role of chemistry in our fundamental understanding of biology, 2056-452X . - Henry Stewart talks. Biomedical & life sciences collection. Chemical biology : the role of chemistry in our fundamental understanding of biology. .
Animated audio-visual presentation with synchronized narration. Title from title frames.
Contents: How do you identify the molecular target of a small molecule drug? -- Flexible regions are key players -- P-loop of ATP-binding proteins is an important example -- Can display libraries of flexible peptides on proteins select those that bind to the ligand -- Selection is through an affinity process called biopanning -- Success depends on the diversity of the library -- Diversity depends on how many different peptides are in the library and their relative abundance -- RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides -- Identification of motifs from a population is easy if the motif is very strong -- Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein -- Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol.
Access restricted to subscribers.
Mode of access: World Wide Web.
1717 Henry Stewart Talks
Biology.
Chemistry.