000			02325ngm a2200337 a 4500
001			HST1717_1_2
003			UkLoHST
006			m c
007			cr|cna|||a||||
007			vz|czazum
008			080121s2008 enk|||||||||||s|||v|eng d
028	5	0	_a1717 _bHenry Stewart Talks
035			_a(UkLoHST)948
035			_a(UkLU-K)AC000393605
040			_aUkLU-K _beng _cUkLU-K
100	1		_aMakowski, Lee. _4spk
245	1	0	_aMapping small molecule binding sites with phage display technology _h[electronic resource] / _cLee Makowski.
260			_aLondon : _bHenry Stewart Talks, _c2008.
300			_a1 online resource (1 streaming video file (39 min.) : _bcolor, sound).
490	1		_aChemical biology : the role of chemistry in our fundamental understanding of biology, _x2056-452X
500			_aAnimated audio-visual presentation with synchronized narration.
500			_aTitle from title frames.
505	0		_aContents: How do you identify the molecular target of a small molecule drug? -- Flexible regions are key players -- P-loop of ATP-binding proteins is an important example -- Can display libraries of flexible peptides on proteins select those that bind to the ligand -- Selection is through an affinity process called biopanning -- Success depends on the diversity of the library -- Diversity depends on how many different peptides are in the library and their relative abundance -- RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides -- Identification of motifs from a population is easy if the motif is very strong -- Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein -- Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol.
506			_aAccess restricted to subscribers.
538			_aMode of access: World Wide Web.
650		2	_aBiology.
650		2	_aChemistry.
830		0	_aHenry Stewart talks. _pBiomedical & life sciences collection. _pChemical biology : the role of chemistry in our fundamental understanding of biology.
856	4	0	_uhttps://hstalks.com/bs/948/
856	4	2	_uhttps://hstalks.com/bs/p/279/ _3Series
999			_c78389 _d78389