TY  - ADVS
AU  - Makowski,Lee
TI  - Mapping small molecule binding sites with phage display technology
T2  - Chemical biology : the role of chemistry in our fundamental understanding of biology,
PY  - 2008///
CY  - London
PB  - Henry Stewart Talks
KW  - Biology
KW  - Chemistry
N1  - Animated audio-visual presentation with synchronized narration; Title from title frames; Contents: How do you identify the molecular target of a small molecule drug? -- Flexible regions are key players -- P-loop of ATP-binding proteins is an important example -- Can display libraries of flexible peptides on proteins select those that bind to the ligand -- Selection is through an affinity process called biopanning -- Success depends on the diversity of the library -- Diversity depends on how many different peptides are in the library and their relative abundance -- RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides -- Identification of motifs from a population is easy if the motif is very strong -- Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein -- Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol; Access restricted to subscribers
UR  - https://hstalks.com/bs/948/
UR  - https://hstalks.com/bs/p/279/
ER  -