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Practical Fluorescence Spectroscopy [electronic resource].

By: Gryczynski, Zygmunt [author.]Material type: TextTextPublisher: Boca Raton : CRC Press LLC, 2019Description: 1 online resource (793 p.)ISBN: 9781439821701; 1439821704; 9781315374758; 1315374757; 9781315320229; 1315320223; 9781498752954; 1498752950Subject(s): MEDICAL / Biochemistry | SCIENCE / Chemistry / Industrial & Technical | SCIENCE / Physics | Fluorescence spectroscopyDDC classification: 543.56 LOC classification: QD96.F56Online resources: Taylor & Francis | OCLC metadata license agreement
Contents:
Cover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Preface -- Authors -- Chapter 1: Theory of Light and Light Interaction with Matter -- 1-1 BASICS OF LIGHT -- 1-2 LIGHT POLARIZATION -- Polarizers -- 1-3 INTERACTION OF LIGHT AND CHROMOPHORES -- Absorption of Light -- Emission of Light -- 1-4 INTERACTIONS AMONG CHROMOPHORES -- Fluorescence Quenching -- Fluorescence (Förster) Resonance Energy Transfer (FRET) -- 1-5 SCATTERING -- Elastic Scattering -- Raman Scattering -- SUMMARY -- Chapter 2: Experimental Basics -- 2-1 TYPES OF CUVETTES -- 2-2 TYPES OF FILTERS
2-3 MEASURING TRANSMITTANCE/ABSORBANCE -- Special Cases of Measuring Absorption -- Measurements of Absorption of Low Volume Samples -- Measurement of Absorption of Small (Solid) Samples -- 2-4 FACTORS AFFECTING THE PRECISION OF ABSORPTION MEASUREMENT -- 2-5 EFFECT OF SAMPLE SCATTERING ON MEASURED TRANSMISSION AND ABSORBANCE -- 2-6 MEASURING EMISSION -- 2-7 EFFICIENCY OF COLLECTING EMISSION: MEASURING QUANTUM YIELD -- Quantum Yield Calculation -- How to Convert from Wavenumbers to Wavelengths? -- Absorption -- Area under the Emission Spectra -- 2-8 WAVELENGTH CALIBRATION -- 2-9 INSTRUMENT RESPONSE
2-10 LAMP PROFILE -- 2-11 PEAKS AND SPECTRAL SHAPES -- 2-12 STANDARD DEVIATION AND HEIGHT -- 2-13 SUMS OF PEAKS -- 2-14 NOISE-SIGNAL-TO-NOISE RATIO -- 2-15 SAMPLING RATE -- 2-16 BANDWIDTH -- 2-17 ARTIFACTS AND ERRORS -- 2-18 SPECTRAL OVERLAP -- 2-19 PEAK NORMALIZATION -- 2-20 RESIDUALS -- 2-21 INTERPOLATION -- Chapter 3: Steady State Experiments-Transmission/Absorption -- INTRODUCTION -- Introduction to Transmittance/Absorbance -- Introduction to Absorption Measurements for Chromophores in Solutions -- Measuring Absorption of Thin Samples (Films) -- EXPERIMENTS
Experiment 3-1 Transmittance of a Transparent Plate -- Experiment 3-2 Transmittance through Irregular (Not Square) Objects -- Experiment 3-3 Transmission of Different Cuvettes -- Experiment 3-4 Transmittance of Cuvette Filled with Air, Water, or Other Solvents-Reflections -- Experiment 3-5 Measuring Absorbance of Chromophores in Solution -- Experiment 3-6 Measuring Absorbance of Chromophores in Solutions: Absorbance Presented in Different Scales -- Experiment 3-7 Absorbance of Rhodamine and Anthracene-Normalized Absorption Spectra
Experiment 3-8 Absorbance of Rhodamine 800-Effect of Out of Range Absorption -- Experiment 3-9 Absorbance of Anthracene-Low Absorption Range -- Experiment 3-10 Low Absorbance-How to Improve Quality of Measured Spectrum -- Experiment 3-11 Absorbance of Rhodamine B-Proper Use of Cuvettes -- Experiment 3-12 How to Adopt a Fluorescence Cuvette for Absorbance Measurement -- Experiment 3-13 Determining the Concentration of Chromophores in a Solution from Known Optical Density (Absorption) -- Experiment 3-14 Absorbance of Benzene-Resolvable Vibrionic States
Summary: Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements
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Description based upon print version of record.

Cover -- Half Title -- Title Page -- Copyright Page -- Table of Contents -- Preface -- Authors -- Chapter 1: Theory of Light and Light Interaction with Matter -- 1-1 BASICS OF LIGHT -- 1-2 LIGHT POLARIZATION -- Polarizers -- 1-3 INTERACTION OF LIGHT AND CHROMOPHORES -- Absorption of Light -- Emission of Light -- 1-4 INTERACTIONS AMONG CHROMOPHORES -- Fluorescence Quenching -- Fluorescence (Förster) Resonance Energy Transfer (FRET) -- 1-5 SCATTERING -- Elastic Scattering -- Raman Scattering -- SUMMARY -- Chapter 2: Experimental Basics -- 2-1 TYPES OF CUVETTES -- 2-2 TYPES OF FILTERS

2-3 MEASURING TRANSMITTANCE/ABSORBANCE -- Special Cases of Measuring Absorption -- Measurements of Absorption of Low Volume Samples -- Measurement of Absorption of Small (Solid) Samples -- 2-4 FACTORS AFFECTING THE PRECISION OF ABSORPTION MEASUREMENT -- 2-5 EFFECT OF SAMPLE SCATTERING ON MEASURED TRANSMISSION AND ABSORBANCE -- 2-6 MEASURING EMISSION -- 2-7 EFFICIENCY OF COLLECTING EMISSION: MEASURING QUANTUM YIELD -- Quantum Yield Calculation -- How to Convert from Wavenumbers to Wavelengths? -- Absorption -- Area under the Emission Spectra -- 2-8 WAVELENGTH CALIBRATION -- 2-9 INSTRUMENT RESPONSE

2-10 LAMP PROFILE -- 2-11 PEAKS AND SPECTRAL SHAPES -- 2-12 STANDARD DEVIATION AND HEIGHT -- 2-13 SUMS OF PEAKS -- 2-14 NOISE-SIGNAL-TO-NOISE RATIO -- 2-15 SAMPLING RATE -- 2-16 BANDWIDTH -- 2-17 ARTIFACTS AND ERRORS -- 2-18 SPECTRAL OVERLAP -- 2-19 PEAK NORMALIZATION -- 2-20 RESIDUALS -- 2-21 INTERPOLATION -- Chapter 3: Steady State Experiments-Transmission/Absorption -- INTRODUCTION -- Introduction to Transmittance/Absorbance -- Introduction to Absorption Measurements for Chromophores in Solutions -- Measuring Absorption of Thin Samples (Films) -- EXPERIMENTS

Experiment 3-1 Transmittance of a Transparent Plate -- Experiment 3-2 Transmittance through Irregular (Not Square) Objects -- Experiment 3-3 Transmission of Different Cuvettes -- Experiment 3-4 Transmittance of Cuvette Filled with Air, Water, or Other Solvents-Reflections -- Experiment 3-5 Measuring Absorbance of Chromophores in Solution -- Experiment 3-6 Measuring Absorbance of Chromophores in Solutions: Absorbance Presented in Different Scales -- Experiment 3-7 Absorbance of Rhodamine and Anthracene-Normalized Absorption Spectra

Experiment 3-8 Absorbance of Rhodamine 800-Effect of Out of Range Absorption -- Experiment 3-9 Absorbance of Anthracene-Low Absorption Range -- Experiment 3-10 Low Absorbance-How to Improve Quality of Measured Spectrum -- Experiment 3-11 Absorbance of Rhodamine B-Proper Use of Cuvettes -- Experiment 3-12 How to Adopt a Fluorescence Cuvette for Absorbance Measurement -- Experiment 3-13 Determining the Concentration of Chromophores in a Solution from Known Optical Density (Absorption) -- Experiment 3-14 Absorbance of Benzene-Resolvable Vibrionic States

Experiment 3-15 Measuring the Transmission of Filters

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

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